BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules: Molecular Cloning, Preparation, and In Vitro Analysis
Figure 8
Time-course profile of transgene expression of AP-PEG-A microencapsulated H9c2 cells transduced with Bac-MGFP under optimal transduction condition. The average fluorescein-expressed values (excitation 485 nm, emission 535 nm) were normalized to that of cells with 24 hpt having 6 h of viral incubation in presence of PBS (taken as 100%) and are represented as normalized mean expression in percentage value (a). The data represent the mean of three different fields, and error bars represent standard error of the mean. Representative photographs of corresponding transduced microencapsulated cells were taken using fluorescence microscope under 100x magnification at different days after encapsulation (b). The white-dotted circles show the peripheral surface of the capsules.
