Enhanced Muscle Growth by Plasmid-Mediated Delivery of Myostatin Propeptide

<table>Schematic illustration representing plasmids used in this study. The mutant myostatin propeptide (MProD76A) gene was cloned into the pCIneo plasmid containing a CMV promoter, chimeric intron and simian virus 40 (SV40) poly (A) sequence. The <svg height="13.175" id="M7" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 13.175" width="12.2875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,13.175)">
<g transform="translate(72,-61.46)">
<text transform="matrix(1,0,0,-1,-71.95,61.69)">
<tspan style="font-size: 12.50px; " x="0" y="0">5</tspan>
</text>
<text transform="matrix(1,0,0,-1,-65.7,67.01)">
<tspan style="font-size: 8.75px; " x="0" y="0"></tspan>
</text>
</g>
</g>
</svg> primer (<svg height="7.125" id="M8" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 19.8125 7.125" width="19.8125" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.125)">
<g transform="translate(72,-66.3)">
<text transform="matrix(1,0,0,-1,-69.87,66.35)">
<tspan style="font-size: 12.50px; " x="0" y="0">→</tspan>
</text>
</g>
</g>
</svg>) and the <svg height="12.9875" id="M9" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 12.2875 12.9875" width="12.2875" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,12.9875)">
<g transform="translate(72,-61.61)">
<text transform="matrix(1,0,0,-1,-71.95,61.84)">
<tspan style="font-size: 12.50px; " x="0" y="0">3</tspan>
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<tspan style="font-size: 8.75px; " x="0" y="0"></tspan>
</text>
</g>
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</svg> primer (<svg height="7.1374998" id="M10" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 18.9375 7.1374998" width="18.9375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,7.1375)">
<g transform="translate(72,-66.29)">
<text transform="matrix(1,0,0,-1,-71.95,66.34)">
<tspan style="font-size: 12.50px; " x="0" y="0">←</tspan>
</text>
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</svg>) are in the coding area of MProD76A and EGFP, respectively. A 510-bp band indicated mRNA expression of MProD76A-EGFP. A 300-bp band indicated mRNA expression of control EGFP.</table>

BioMed Research International

fig1

Figure 1

Figure 1: Enhanced Muscle Growth by Plasmid-Mediated Delivery of Myostatin Propeptide 