Enhanced Muscle Growth by Plasmid-Mediated Delivery of Myostatin Propeptide
Figure 3
In vivo expression of pCI-MProD76A-EGFP in injected mice. (a) Expression of pCI-MProD76A-EGFP assayed by RT-PCR after intramuscular injection of pCI-MProD76A-EGFP into the quadriceps muscle of mice. Left panels: RT-PCR bands (510 bp) for MProD76A-EGFP; RT-PCR bands (350 bp) for -actin, which served as an internal control. Right panels: RT-PCR bands for EGFP (300 bp) and endogeous myostatin (460 bp). M, DNA markers; C, control muscle; MG, pCI-MProD76A-EGFP-injected muscle; G, pCI-EGFP-injected muscle. (b) The serum levels of the EGFP protein (ng/mL) in mice injected with pCI-MProD76A-EGFP or pCI-EGFP. The results are presented as means ± SEM, .
