Enhanced Muscle Growth by Plasmid-Mediated Delivery of Myostatin Propeptide

<table>Effects of myostatin propeptide delivery on growth performance of mice. <svg height="13.575" id="M41" style="vertical-align:-2.21957pt" version="1.1" viewbox="0 0 49.412498 13.575" width="49.412498" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,13.575)">
<g transform="translate(72,-61.14)">
<text transform="matrix(1,0,0,-1,-71.95,63.4)">
<tspan style="font-size: 12.50px; " x="0" y="0">(</tspan>
<tspan style="font-size: 12.50px; " x="4.1634989" y="0">𝑛</tspan>
<tspan style="font-size: 12.50px; " x="13.840821" y="0">=</tspan>
<tspan style="font-size: 12.50px; " x="25.88121" y="0">6</tspan>
<tspan style="font-size: 12.50px; " x="32.13271" y="0">)</tspan>
<tspan style="font-size: 12.50px; " x="36.296207" y="0">.</tspan>
</text>
</g>
</g>
</svg> Four-week-old mice were injected with pCI-MProD76A-EGFP or pCI-EGFP into the regenerating quadriceps muscle. (a) Mice injected with pCI-MProD76A-EGFP showed a significant increase in body mass compared with pCI-EGFP control mice <svg height="15.4375" id="M42" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 71.599998 15.4375" width="71.599998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,15.4375)">
<g transform="translate(72,-59.65)">
<text transform="matrix(1,0,0,-1,-71.95,62.83)">
<tspan style="font-size: 12.50px; " x="0" y="0">(</tspan>
</text>
<text transform="matrix(1,0,0,-1,-65.7,67.83)">
<tspan style="font-size: 8.75px; " x="0" y="0">∗</tspan>
</text>
<text transform="matrix(1,0,0,-1,-60.63,61.87)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
<tspan style="font-size: 12.50px; " x="10.9" y="-0.95999998">&lt;</tspan>
<tspan style="font-size: 12.50px; " x="22.927885" y="-0.95999998">.</tspan>
<tspan style="font-size: 12.50px; " x="26.053637" y="-0.95999998">0</tspan>
<tspan style="font-size: 12.50px; " x="32.305138" y="-0.95999998">5</tspan>
<tspan style="font-size: 12.50px; " x="38.556637" y="-0.95999998">)</tspan>
<tspan style="font-size: 12.50px; " x="42.720135" y="-0.95999998">.</tspan>
</text>
</g>
</g>
</svg> (b) The mass of individual hindlimb muscles is increased in mice injected with pCI-MProD76A-EGFP (<svg height="15.4375" id="M43" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 63 15.4375" width="63" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,15.4375)">
<g transform="translate(72,-59.65)">
<text transform="matrix(1,0,0,-1,-69.87,67.83)">
<tspan style="font-size: 8.75px; " x="0" y="0">∗</tspan>
<tspan style="font-size: 8.75px; " x="4.5772958" y="0">∗</tspan>
</text>
<text transform="matrix(1,0,0,-1,-60.21,61.87)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
<tspan style="font-size: 12.50px; " x="10.9" y="-0.95999998">&lt;</tspan>
<tspan style="font-size: 12.50px; " x="22.927885" y="-0.95999998">.</tspan>
<tspan style="font-size: 12.50px; " x="26.053637" y="-0.95999998">0</tspan>
<tspan style="font-size: 12.50px; " x="32.305138" y="-0.95999998">1</tspan>
</text>
</g>
</g>
</svg>; <svg height="15.4375" id="M44" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 66.387497 15.4375" width="66.387497" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,15.4375)">
<g transform="translate(72,-59.65)">
<text transform="matrix(1,0,0,-1,-69.87,67.83)">
<tspan style="font-size: 8.75px; " x="0" y="0">∗</tspan>
</text>
<text transform="matrix(1,0,0,-1,-64.79,61.87)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
<tspan style="font-size: 12.50px; " x="10.9" y="-0.95999998">&lt;</tspan>
<tspan style="font-size: 12.50px; " x="22.927885" y="-0.95999998">.</tspan>
<tspan style="font-size: 12.50px; " x="26.053637" y="-0.95999998">0</tspan>
<tspan style="font-size: 12.50px; " x="32.305138" y="-0.95999998">5</tspan>
<tspan style="font-size: 12.50px; " x="38.556637" y="-0.95999998">)</tspan>
<tspan style="font-size: 12.50px; " x="42.720135" y="-0.95999998">.</tspan>
</text>
</g>
</g>
</svg> (c) Comparison of the heart, the liver, the kidney, and the epididymal fat pad weights, no significant difference was found between the two groups.</table>

BioMed Research International

fig4

Figure 4

Figure 4: Enhanced Muscle Growth by Plasmid-Mediated Delivery of Myostatin Propeptide 