Figure 4: Induction of macrophage marker expression on WEHI-3B JCS cells by rSj16. WEHI-3B JCS cells were treated for 72  hours in the presence of 0.5  𝜇 g/mL rSj16 (rSj16), 0.5  𝜇 g/mL rGST (rGST), or medium alone (untreated) and then analyzed for expression of F4/80 and Gr-1 using flow cytometry. (a) Red line indicates isotype-matched control antibody; black line, F4/80 or Gr-1. (b) Column indicates mean ± SEM of four independent experiments. 𝑃 < . 0 5 as compared with that of untreated group.