BioMed Research International / 2010 / Article / Fig 7

Research Article

Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages

Figure 7

Effect of rSj16 on IL-3, M-CSF, or G-CSF induced colony formation of mouse bone marrow cells. Mouse bone marrow cells were plated in 0.33% agar cultures containing G-CSF (a), M-CSF (b), or IL-3 (c) in the presence of rSj16, rGST, or PBS in 24-well plates. The agar gels were dried and stained with hematoxylin solution following seven days of incubation. Differential colony counts were performed based on the morphology of the cells, and only colonies of greater than 40 cells were counted. Colony counts are indicated as number of colonies per well. Data are mean ± SEM of triplicate wells and are representatives of three individual experiments. 𝑃 < . 0 5 ; 𝑃 < . 0 1 ; NS, not significant versus the corresponding data in the PBS + G-CSF/M-CSF/IL-3 group.

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