Figure 1: mRNA expression profile of E. histolytica putative ESCRT machinery genes. (a) RT-PCR products obtained from 1  𝜇 g of total RNA from trophozoites growing in TYI-S-33 medium (0 minute) or after 5 minutes of erythrophagocytosis. (b) Densitometric analysis of RT-PCR products in (a). Pixels corresponding to Eh25S rRNA amplified product were taken as 100% in each lane. Data represent the mean of three independent experiments performed by duplicate for each gene. Asterisk, genes whose transcription is significantly changed according to 𝑇 Student test.