Detection of the Endosomal Sorting Complex Required for Transport in Entamoeba histolytica and Characterization of the EhVps4 Protein
Figure 6
Cellular localization of exogenous EhVps4 in transfected trophozoites. Trophozoites transfected with pNEO ((a) and (b)), pEhVps4 ((c) and (d)) and pEhVps4-E211Q ((e) and (f)) were incubated with mouse anti-FLAG antibodies, treated with FITC-labeled secondary antibodies, counterstained with DAPI and analyzed through confocal laser microscopy. pEhVps4-E211Q transfected trophozoites incubated with FITC-labeled secondary antibodies were used as control ((g) and (h)). (a), (c), (e) and (g) Cells observed in phase contrast; (b), (d), (f) and (h) Merge, trophozoites observed in the green (FITC) and blue (DAPI) channels. Arrowheads, EhVps4 signal in plasma membrane and cytoplasmic dots.
