Detection of the Endosomal Sorting Complex Required for Transport in Entamoeba histolytica and Characterization of the EhVps4 Protein

Table 1

Comparison of 𝐸𝑛𝑡𝑎𝑚𝑜𝑒𝑏𝑎ℎ𝑖𝑠𝑡𝑜𝑙𝑦𝑡𝑖𝑐𝑎, 𝐻𝑜𝑚𝑜𝑠𝑎𝑝𝑖𝑒𝑛𝑠, and 𝑆𝑎𝑐𝑐ℎ𝑎𝑟𝑜𝑚𝑦𝑐𝑒𝑠𝑐𝑒𝑟𝑒𝑣𝑖𝑠𝑖𝑎𝑒 ESCRT machineries.

Entamoebahistolytica			Homosapiens					Saccharomycescerevisiae
Putative complex	Predicted protein	Accesionnumber^a	Protein	Accesion number^b	𝑒-value	S (%)	I (%)	Protein	Accesion number^a	𝑒-value	S (%)	I (%)
ESCRT-0 complex	EhHse1	EHI_091530	STAM1	Q92783	9𝑒−08	72	50	Hse1p	P38753	2𝑒−10	70	62
	EhVps27	EHI_117910	HRS	O14964	—	—	—	Vps27p	P40343	1𝑒−11	61	49
ESCRT-I complex	EhVps23	EHI_135460	TSG101	Q99816	1.4𝑒−10	51	33	Vps23p	P25604	0.0052	31	25
	EhVps28	EHI_108630	hVps28	Q9UK41	—	—	—	Vps28p	Q02767	0.00057	45	28
	EhVps37A	EHI_077870	hVps37A	Q6NW27	0.00022	55	24	Vps37p	Q99176	0.00084	46	21
	EhVps37D	EHI_060400	hVps37D	Q6P2C3	3.8𝑒−06	50	35	nd	—	—	—	—
	nd	—	nd	—	—	—	—	Mvb12	P42939	—	—	—
ESCRT-II complex	EhVps22	EHI_131120	EAP30	Q96H20	9𝑒−22	47	30	Vps22p	Q12483	3.4𝑒−15	48	25
	EhVps25	EHI_137860	EAP20	Q9BRG1	9𝑒−08	52	28	Vps25p	P47142	0.00038	46	19
	EhVps36	EHI_045320	EAP45	Q86VN1	1.8𝑒−15	49	25	Vps36p	Q06696	1.1𝑒−09	48	26
ESCRT-III complex	EhVps2	EHI_194400	CHMP2A	O43633	8.9𝑒−24	55	29	Vps2p	P36108	2𝑒−05	50	29
			CHMP2B	Q9UQN3	7.9𝑒−16	48	25	—	—	—	—	—
	EhVps20	EHI_066730	CHMP6	Q96FZ7	—	—	—	Vps20p	Q04272	0.00015	54	26
	EhVps24	EHI_048690	CHMP3	Q9Y3E7	—	—	—	Vps24p	P36095	2.2𝑒−05	48	22
	EhVps32	EHI_169820	CHMP4A	Q9BY43	0.0012	48	24	Vps32p	P39929	2.5𝑒−12	48	25
			CHMP4B	Q9H444	9.6𝑒−07	43	20	nd	—	—	—	—
			CHMP4C	Q96CF2	1.4𝑒−06	43	20	nd	—	—	—	—
Vps4	EhVps4	EHI_118900	hVps4A	Q9UN37	1.1𝑒−114	69	52	Vps4p	P52917	3𝑒−114	78	60
			hVps4B	O75351	3𝑒−114	78	60	—	—	—	—	—
Other MVB proteins	EhVta1	EHI_010040	hVta1	Q9NP79	2.8𝑒−05	44	23	Vta1p	Q06263	—	—	—
	EhVps46	EHI_093850	CHMP1A	Q9HD42	—	—	—	Vps46p	P69771	0.00032	48	18
			CHMP1B	Q7LBR1	—	—	—	nd	—	—	—	—
	EhVps60	EHI_114790	CHMP5	Q9NZZ3	5.6𝑒−15	45	28	Vps60p	Q03390	9𝑒−06	46	19
	EhDoa4	EHI_012290	UBP4	Q13107	1𝑒−77	58	36	Doa4p	P32571	2𝑒−27	50	31
	EhADH112	EHI_181220	ALIX	Q9UKL5	1𝑒−21	40	20	Rim20p	Q12033	2𝑒−12	38	21
								BRO1p	P48582	0.003	50	32
Ubiquitination components	nd	—	nd	—	—	—	—	Bul1p	P48524	—	—	—
	EhRsp5	EHI_011530	NEED4	P46934	1.6𝑒−50	53	33	Rsp5p	P39940	7.8𝑒−57	57	36

^aPathema Entamoeba Bioinformatics Resource Center. ^bSwiss-Prot/TrEMBL databases. nd: not determined. —: Denote nonsignificant similarity/identity and 𝑒-values. S: Similarity. I: Identity.