BioMed Research International

Research Article

Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in Human and Other Primate Cell Lines

Table 2

EBV-PCR+ cell lines, BZLF1 expression detected by Western blotting, differentiation of episomal and linear EBV-DNA, EBV sequence pattern detected by Southern blotting, and episomal load determined by FISH.


Cell line	Cell type	PCR^a	BZLF1 expr.^a		In situ lysing gel^b		Southern blot^c	FISH^d
Cell line	Cell type	PCR^a	untr.	TPA	episomal	linear	Southern blot^c	FISH^d

ARH-77	B-LCL	+	−	−	(+)	−	+	+
B95-8	marmoset monkey lymphocytes	+	+	+	++	+++	+	+++
BD-215	B-LCL	+	+	+	+	+	+	++
BONNA-12	hairy cell leukemia	+	−	−	++	+	+	++ (h)
CI-1	B cell lymphoma	+	−	−	+	−	n.d.	+
COLO-720L	B-LCL	+	−	−	+	+	+	++
CRO-AP2	B cell lymphoma	+	−	−	(+)	−	+	++
CRO-AP5	B cell lymphoma	+	−	−	(+)	−	+	++
DAUDI	Burkitt lymphoma	+	−	−	+++	+	+	++ (h)
DOHH-2	B cell lymphoma	+	−	−	−	−	−	+ (f)
EB-1	Burkitt lymphoma	+	−	+	+	+	+	++ (h)
EHEB	chronic B cell leukemia	+	−	−	+	(+)	+	n.d.
GRANTA-519	B cell lymphoma	+	−	+	++	++	+	++
HC-1	hairy cell leukemia	+	−	+	++	(+)	+	++++
IM-9	B-LCL	+	−	+	+++	−	+	++++
JIYOYE	Burkitt lymphoma	+	−	+	++	++	+	+++
JVM-13	chronic B cell leukemia	+	−	−	++ (2 bands)	−	+	++++
JVM-2	chronic B cell leukemia	+	−	−	+	+	+	+++
JVM-3	chronic B cell leukemia	+	−	−	++	+	+	++
L-591	B-LCL	+	+	+	++	++	+	+++
LCL-HO	B-LCL	+	−	−	++	(+)	+	+++
LCL-WEI	B-LCL	+	−	−	+	+	+	++
MEC-1	chronic B cell leukemia	+	−	−	+	+	+	++ (h)
MEC-2	chronic B cell leukemia	+	−	−	+	−	+	++ (h)
NAMALWA	Burkitt lymphoma	+	−	−	−	−	(+)	−
NAMALWA.CSN/70	Burkitt lymphoma	+	−	−	−	−	(+)	−
NAMALWA.IPN/45	Burkitt lymphoma	+	−	−	−	−	(+)	−
NAMALWA.KN2	Burkitt lymphoma	+	−	−	−	−	(+)	−
NAMALWA.PNT	Burkitt lymphoma	+	−	−	−	−	(+)	−
NC-NC	B-LCL	+	+	+	++	++	+	+++
NK-92	natural killer lymphoma	+	−	−	+	+	+	+
OCI-LY-19	B cell lymphoma	+	−	−	−	−	−	−
RAJI	Burkitt lymphoma	+	−	−	+++	+	+	++++
RO	B-LCL	+	−	−	+	−	+	++
SD-1	B-LCL	+	−	−	++	+	+	+++
T2	human-human somatic cell hybrid	+	−	−	++	+	n.d.	n.d.
TMM	B-LCL	+	−	−	+ (2 bands)	(+)	+	+++
VAL	B cell lymphoma	+	−	+	++ (2 bands)	+	n.d.	++ (h)
YT	T/natural killer cell leukemia	+	+	+	++	+++	+	+++

B-LCL: B-lymphoblastoid cell line; expr.: expression; n.d.: not determined; untr.: untreated.
^a−: negative; +: positive.
^b−: negative; (+): weakly positive; +: positive; ++: strongly positive; +++: very strongly positive.
^c −: no bands visible; (+): very weak signals; +: clear bands visible.
^d episomal load detected by FISH: −: no episomes; +: low load; ++: load; +++: high load; ++++: very high load; (h): heterogenous distribution of the episomes among the cells; (f): only a few cells containing episomes.