Induction by TNF-𝛼 of IL-6 and IL-8 in Cystic Fibrosis Bronchial IB3-1 Epithelial Cells Encapsulated in Alginate Microbeads

Table 1

Experimental design matrix and results of the DoE (design of experiments) approach for alginate microbeads.

		Factors		Responses
Batch #	Atomizing air (bar)	Distance between the nozzle and the gelling bath (cm)	Polymer pumping rate Pump (𝜇m/mL)	Mean diameter (𝜇m)	Standard deviation (±𝜇m)
	“air”	“height”	“pump”	“size”	“SD”
#1	12.0	2.2	75.0	1350.5	63.2
#2	20.0	2.2	75.0	983.1	68.4
#3	12.0	3.0	75.0	1234.1	389.5
#4	20.0	3.0	75.0	917.5	60.5
#5	12.0	2.2	125.0	1177.5	296.5
#6	20.0	2.2	125.0	716.7	99.2
#7	12.0	3.0	125.0	1161.1	83.6
#8	20.0	3.0	125.0	724.8	106.7
#9	12.0	2.6	100.0	1293.6	47.6
#10	20.0	2.6	100.0	835.4	322.3
#11	16.0	2.2	100.0	970.5	54.8
#12	16.0	3.0	100.0	851.6	36.3
#13	16.0	2.6	75.0	920.6	47.2
#14	16.0	2.6	125.0	974.9	58.1
#15a	16.0	2.6	100.0	920.8	43.1
#15b	16.0	2.6	100.0	920.8	43.1
#15c	16.0	2.6	100.0	920.8	43.1