The Anti-Inflammatory Activity of HMGB1 A Box Is Enhanced When Fused with C-Terminal Acidic Tail

Figure 4

The C tail-fused A box could more effectively protect against endotoxin lethality. BALB/c mice (20 mice/group), subjected to LD₇₅ doses of LPS, were treated at 0 and 12 hours after LPS administration with A box, A box+C tail, A box+(Gly₄Ser)₃+C tail, or DHFR control proteins (40 nmol/mouse i.p.). Mortality was monitored for up to 10 days. The survival rates were analyzed by the Kaplan-Meier method with comparisons between different groups. The results showed that the A box alone and the two C tail-fused A box proteins could significantly prolong life time and improve the survival rates of the mice (𝑃<.05). The A box+(Gly₄Ser)₃+C tail protein had the most protective effect, followed by A box+C tail and A box (𝑃<.05).