BioMed Research International / 2010 / Article / Tab 1

Methodology Report

A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation

Table 1

Protein recovery after plasma prefractionation using immunoaffinity depletion of high-abundant proteins or hexapeptide ligand library treatment. Protein content was estimated using FluoroProfile Protein Quantification Kit.

MARS-Hu 14Proteo Miner

Plasma volume loaded ( ๐œ‡ L)10 ร— 10 depletion cycles900
Protein quantity loaded (mg) (whole plasma : 63โ€‰mg/mL proteins)6,356,7
Protein quantity recovered after prefractionation ( ๐œ‡ g) 3 4 0 4 0 1 4 1 5 2 9 9 1 4 2 0 1 1 5 8 1 1 9 5 1 5 7 2
Average protein quantity recovered after prefractionation ( ๐œ‡ g)364 (or 36.4โ€‰ ๐œ‡ g per depletion cycle)1336
% of total protein mass removal by prefractionation94.297.6