One-DE of 15 g of mycelium protein extract of six different strains of B. cinerea (B05.10, CECT 2100, CECT 2850, CECT 2996, CECT 20518, BOLC (isolated from infected lentil plants)). This approach allowed us to observe differences in the protein band patterns among strains. The bands were cut out and the protein identification was made using MALDI-TOF/TOF MS/MS, and PMF search and a combined search (+MS/MS) were performed in nrNCBI database of proteins using MASCOT. Some of these proteins identified have been reported to be involved in pathogenicity in B. cinerea or in other phytopathogenic fungi, such as malate dehydrogenase (10), woronin body major protein (11), peptidyl-prolyl cis-trans isomerase (14) and PIC5 protein (15), or implicated in fungal growth and differentiation, such as nucleoside diphosphate kinase (12). The abundance of these proteins was different among isolated (Gonzalez-Fernandez et al., unpublished results).
