Characterization of Recombinant Dengue-2 Virus Derived from a Single Nucleotide Substitution in the 5′ Noncoding Region

Table 4

Percent Infection and Dissemination in generation 1–5.

Viruses	D2-16681		D2-14.5^M		D2-15.5^M		D2-57.5^M
	Infection (%)^a	Dissemination (%)^b	Infection (%)	Dissemination (%)	Infection (%)	Dissemination (%)	Infection (%)	Dissemination (%)
G1	31 (62%)	24 (77%)	19 (38%)	10 (53%)	21 (42%)	12 (57%)	16 (32%)	7 (44%)
G2	42 (48%)	20 (47%)	16 (32%)	5 (31%)	23 (46%)	10 (43%)	15 (30%)	5 (33%)
G3	37 (74%)	15 (40%)	18 (36%)	7 (39%)	17 (34%)	5 (29%)	5 (10%)	3 (60%)
G4	20 (40%)	14 (70%)	16 (32%)	7 (44%)	19 (38%)	5 (26%)	9 (18%)	1 (11%)
G5	18 (36%)	6 (33%)	12 (24%)	4 (33%)	8 (16%)	3 (38%)	4 (8%)	2 (50%)

^anumber of IFA positive mosquitoes in gut part/number of mosquitoes (𝑛=50)×100.
^bnumber of IFA positive mosquitoes in head part/number of IFA positive mosquitoes in gut part × 100.