JAK3/STAT5/6 Pathway Alterations Are Associated with Immune Deviation in CD𝟖⁺ T Cells in Renal Cell Carcinoma Patients

Table 2

Flow cytometry analysis of CD45RA/CCR7 expression on CD8⁺ T cells at day 0 and after 35 days of MLTC. Differential expression of the CD45RA, CCR7 cell surface molecules was analyzed on CD8⁺ T cells at day 0 and after extended MLTC stimulation. CD8⁺ T cells were separated into four subsets (𝑇_𝑁, 𝑇_𝐶𝑀,𝑇_𝐸𝑀,𝑇_{𝐸𝑀𝑅𝐴}) based on differential expression of the CD45RA and CCR7 markers. Results are the mean (+/− SD) of values obtained from 5 RCC patients and their corresponding HLA-matched normal donors.

	CD8^+ Naive		TCM		TEM		TEMRA
	CD45RA^+CCR7^+		CD45RA^−CCR7^+		CD45RA^−CCR7^−		CD45RA^+CCR7^−
	Day 0	Day 35	Day 0	Day 35	Day 0	Day 35	Day 0	Day 35
RCC patients	30.6 ± 4.0	4.4 ± 1.6	8.2 ± 2.3	11 ± 2.8	14 ± 3.8	52.6 ± 6.7	46 ± 5.3	30.6 ± 4.2
Healthy donors	49 ± 3.1	3.1 ± 1.6	10.8 ± 5.3	59 ± 4.6	20.2 ± 3.9	32.8 ± 7.2	17.4 ± 3.6	4.4 ± 1.8
𝑃 value	.021	.139	.019	.008	.034	.003	.0017	.0006