BioMed Research International / 2010 / Article / Tab 2

Research Article

Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids

Table 2

Mass spectrometry-detected peptides found among all pertussis toxins and toxoids.

Peptides DetectedToxin AToxin BToxoid CToxoid D

S1DDPPATVYR (35–43) XXX
SCQVGSSNSAFVSTSSSR (74–91) X X
YTEVYLEHR (93–101)XXXX
MQEAVEAER (102–110)XXXX
GTGHFIGYIYEVR (114–126)XXXX
ADNNFYGAASSYFEYVDTYGDNAGR (127–151)XXXX
ILAGALATYQSEYLAHR (152168)XXXX
IPPENIR (170176)XXXX
VYHNGITGETTTTEYSNAR (181–199)XXX
YVSQQTRANPNYTSR (200–215)X
ANPNPYTSR (207–215) X
SVASIVGTLVR (217227)XXX X
QAESSEAMAAWSER (239252)XXXX

S2ALTVAELR (56–63) XXX
GSGDLQEYLR (6473)XXXX
GWSIFALYDGTYLGGEYGGVIK (78–99)XX
DGTPGGAFDLK (100110)XXXX
NTGQPATDHYYSNVTATR (120–137)XXX
LCAVFVR (146–152)X
SGQPVIGACTSPYDGK (153–168)XXX
MLYLIYVAGISVR (179–191) X

S3ALFTQQGGAYGR (3950)XXXX
ALTVAELR (57–64)XXX
GNAELQTYLR (65–74) XXX
QITPGWSIYGLYDGTYLGQAYGGIIK (75–100)XXX
DAPPGAGFIYR (101111) XXXX
ETFCITTIYK (112121)XXXX
TGQPAADHYYSK (122–133)XXX
LLASTNSR (139–146) X
LCAVFVR (147–153) X
DGQSVIGACASPYEGR (154–169) X
DMYDALR (172–178)X
LLYMIYMSGLAVR (180–192)XX

S4DVPYVLVK (43–50)X
TNMVVTSVAMKPYEVTPTR (5169)XXXX
MLVCGIAAK (70–78)X
LGAAASSPDAHVPFCFGK (79–96) X X
RPGSSPMEVMLR (100111)XXXX
QLTFEGKPALELIR (128–141) X XX

S5NFTVQELALK (43–52) XXX

-Detected peptide present in source (X-box); Detected peptide absent in source (white box)
-Common peptide (detected among all sources)
-Instruments: MALDI-TOF, LTQ-Orbitrap & Q-Tof Premier ( − ) Rapigest