Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids

Table 2

Mass spectrometry-detected peptides found among all pertussis toxins and toxoids.

	Peptides Detected	Toxin A	Toxin B	Toxoid C	Toxoid D
S1	DDPPATVYR (35–43)	X	X		X
	SCQVGSSNSAFVSTSSSR (74–91)		X	X
	YTEVYLEHR (93–101)	X	X	X	X
	MQEAVEAER (102–110)	X	X	X	X
	GTGHFIGYIYEVR (114–126)	X	X	X	X
	ADNNFYGAASSYFEYVDTYGDNAGR (127–151)	X	X	X	X
	ILAGALATYQSEYLAHR (152–168)	X	X	X	X
	IPPENIR (170–176)	X	X	X	X
	VYHNGITGETTTTEYSNAR (181–199)	X	X	X
	YVSQQTRANPNYTSR (200–215)			X
	ANPNPYTSR (207–215)		X
	SVASIVGTLVR (217–227)	X	X	X	X
	QAESSEAMAAWSER (239–252)	X	X	X	X
S2	ALTVAELR (56–63)	X		X	X
	GSGDLQEYLR (64–73)	X	X	X	X
	GWSIFALYDGTYLGGEYGGVIK (78–99)	X	X
	DGTPGGAFDLK (100–110)	X	X	X	X
	NTGQPATDHYYSNVTATR (120–137)	X	X	X
	LCAVFVR (146–152)				X
	SGQPVIGACTSPYDGK (153–168)	X	X	X
	MLYLIYVAGISVR (179–191)	X
S3	ALFTQQGGAYGR (39–50)	X	X	X	X
	ALTVAELR (57–64)	X		X	X
	GNAELQTYLR (65–74)	X		X	X
	QITPGWSIYGLYDGTYLGQAYGGIIK (75–100)		X	X	X
	DAPPGAGFIYR (101–111)	X	X	X	X
	ETFCITTIYK (112–121)	X	X	X	X
	TGQPAADHYYSK (122–133)	X	X	X
	LLASTNSR (139–146)				X
	LCAVFVR (147–153)			X
	DGQSVIGACASPYEGR (154–169)	X
	DMYDALR (172–178)		X
	LLYMIYMSGLAVR (180–192)	X	X
S4	DVPYVLVK (43–50)	X
	TNMVVTSVAMKPYEVTPTR (51–69)	X	X	X	X
	MLVCGIAAK (70–78)		X
	LGAAASSPDAHVPFCFGK (79–96)	X	X
	RPGSSPMEVMLR (100–111)	X	X	X	X
	QLTFEGKPALELIR (128–141)	X	X	X
S5	NFTVQELALK (43–52)	X	X		X

-Detected peptide present in source (X-box); Detected peptide absent in source (white box)
-Common peptide (detected among all sources)
-Instruments: MALDI-TOF, LTQ-Orbitrap & Q-Tof Premier (−) Rapigest