Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids

Table 3

Comparative pertussis toxin and toxoid subunit sequence analysis (−/+ rapigest treatment).

Subunit	Source	− RG; (% AA coverage)	# peptides detected	+ RG; (% AA coverage)	# peptides detected
S1	Toxin A	58	10	57	10
	Toxin B	69	12	74	12
	Toxoid C	49	8	66	10
	Toxoid D	41	7	70	11
S2	Toxin A	33	4	31	4
	Toxin B	39	4	45	7
	Toxoid C	14	2	14	2
	Toxoid D	5	1	14	2
S3	Toxin A	28	5	49	8
	Toxin B	39	6	60	9
	Toxoid C	12	2	38	6
	Toxoid D	18	2	33	5
S4	Toxin A	53	4	28	2
	Toxin B	57	4	41	3
	Toxoid C	28	2	28	2
	Toxoid D	28	2	50	4
S5	Toxin A	—	—	10	1
	Toxin B	—	—	18	2
	Toxoid C	—	—	10	1
	Toxoid D	—	—	10	1

-Approach: Electrospray Ionization Mass Spectrometry-Instrument: LTQ-Orbitrap only-RG (Rapigest)