BioMed Research International / 2010 / Article / Tab 4

Research Article

Mass Spectrometric Analysis of Multiple Pertussis Toxins and Toxoids

Table 4

Peptide detection among pertussis toxin and toxoid subunits ( /+ rapigest treatment).

RG+ RG

S1GTGHFIGYIYEVR (114–126)
GTGHFIGYIYEVR (114–126)YTEVYLEHR (93–101)
YTEVYLEHR (93–101)MQEAVEAER (102–110)
MQEAVEAER (102–110)IPPENIR (170–176)
IPPENIR (170–176)VYHNGITGETTTTEYSNAR (181199)
ILAGALATYQSEYLAHR (152168)
MAPVIGACMAR (228238)

S2GSGDLQEYLR (64–73)GSGDLQEYLR (64–73)
NTGQPATDHYYSNVTATR (120137)

S3ALFTQQGGAYGR (39–50)ALFTQQGGAYGR (39–50)
QITPGWSIYGLYDGTYLGQAYGGIIK (75100)
GNAELQTYLR (6574)
DAPPGAGFIYR (101111)

S4TNMVVTSVAMKPYEVTPTR (51–69)TNMVVTSVAMKPYEVTPTR (51–69)
RPGSSPMEVMLR (100111)

S5NFTVQELALK (4352)

-Peptides detected in +RG (rapigest) (in bold) -Approach: Electrospray Ionization Mass Spectrometry-Instrument: LTQ-Orbitrap only