Proteomic Analysis of Pichindé virus Infection Identifies Differential Expression of Prothymosin-𝛼

Figure 1

SELDI spectra reveal virus and time-based peak differences. Guinea pigs were mock-infected or infected with the P2 or P18 variant of Pichinde virus. At 1 day and 6 days post infection, peritoneal cells were harvested and cytoplasmic and nuclear extracts prepared and analyzed using surface enhanced laser desorption ionization time-of-flight mass spectrometry. Experiments were performed in triplicate. Protein peaks were identified which showed patterns specific to type and stage of infection. The figure shows a representative set of spectra from cytoplasmic extracts.