Expression and Purification of Z Protein from Junín Virus
Figure 5
Western Blotting with α-Tio-Z-V5-His. (a) The ultracentrifugated supernatant (UC pellet) and cellular extract (Cell) of Sf9 cell fractions infected with recombinant v_Ac-Z were loaded in lanes 1 and 2. The same fractions were loaded for uninfected cells (ui, lanes 3 and 4) and control v_dsRed (recombinant baculovirus expressing dsRed protein, lanes 5 and 6). (b) Proteinase K protection assay. The treatment of the UC pellet samples (Triton X-100 1% and Proteinase K 0,1 mg/ml) is indicated on the top of the figure.
