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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 135034, 10 pages
Research Article

The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model

1Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands
2Department of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK
3Department of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy
4Microbiology Section, Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134 Verona, Italy
5DIPTERIS, University of Genoa, Corso Europa 26, 16132 Genoa, Italy
6Department of Cariology, Institute of Odontology at Sahlgrenska Academy, University of Gothenburg, P.O. Box 450, 405 30 Göteborg, Sweden

Received 14 June 2011; Accepted 14 July 2011

Academic Editor: Itzhak Ofek

Copyright © 2011 Egija Zaura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the current study was to investigate the anticariogenic potential of the (sub)fractions obtained from the edible mushroom shiitake (Lentinula edodes) in in vitro caries model. We used a modified constant depth film fermentor (CDFF) with pooled saliva as the inoculum and bovine dentin as a substratum. The test compounds were low molecular weight fraction (MLMW) of the shiitake extract and subfractions 4 and 5 (SF4 and SF5) of this fraction. Chlorhexidine (CHX) and water served as a positive and a negative control, respectively. Dentin mineral loss was quantified (TMR), microbial shifts within the microcosms were determined (qPCR), and the acidogenicity of the microcosms was assessed (CIA). From the compounds tested, the SF4 of shiitake showed strong inhibiting effect on dentin demineralization and induced microbial shifts that could be associated with oral health. The acid producing potential was increased, suggesting uncoupling of the glycolysis of the microbiota by the exposure to SF4. In conclusion, the results suggest that SF4 of shiitake has an anticariogenic potential.