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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 165852, 8 pages
http://dx.doi.org/10.1155/2011/165852
Research Article

Detailed Investigations on the Solid Cell Culture and Antimicrobial Activities of the IranianArnebia euchroma

1Biochemistry Department, The National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, Iran
2Biochemistry Department, Tonekabon Branch, Islamic Azad University, P.O. Box 4684161187, Tonekabon, Iran
3Pharmaceutical Sciences Faculty, Shahid Beheshti University, P.O. Box 14155-6153, Tehran, Iran

Received 15 October 2010; Revised 7 April 2011; Accepted 26 April 2011

Academic Editor: Paul W. Doetsch

Copyright © 2011 K. Haghbeen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In pursuit of strong shikalkin-producing cell lines, seeds of the Iranian Arnebia euchroma were collected from Dena altitudes in the central Zagross. Chemical analysis showed that the dried root of the plant contained about 8.5% (w/w) shikalkin pigment. The root explants of the young plantlets, obtained from the germinated seeds, were used for establishing callus. Then, parameters effective on proliferation and pigment production of the resulting calli were studied in detail. Accordingly, two modified media called mLS and mM9 were optimized for propagation and pigment production, respectively. Using these media, the biomass of the A.euchroma calli was increased to 600%, and the pigment production reached to a maximum of 16.3 mg per gram of the wet biomass in a period of a subculture (21 days). Parallel to these experiments, the antimicrobial activity of shikalkin pigment was examined on some fungi and Gram-positive and Gram-negative bacteria. Results indicated that the pigment was almost ineffective on fungi and Gram-negative bacteria, but it was meaningfully effective against Micrococcus luteus.