Detailed Investigations on the Solid Cell Culture and Antimicrobial Activities of the IranianArnebia euchroma

Figure 3

(a) The growth of 5 A. euchroma calli obtained from the root explants of 5 different seeds and subculturd on LS medium containing 2,4-D (10⁻⁶ M), kinetin (10⁻⁵ M), and sucrose (30 g/L) in the dark (filled bars) and the light (spotted bars). The growth of the A. euchroma callus subculturd on: (b) LS medium containing 2,4-D (10⁻⁶ M), kinetin (10⁻⁵ M), and different amounts of sucrose in the dark, (c) modified LS media [LS (normal LS), LS1 ([NH₄NO₃] = 0, [KNO₃] = 1900 mg/L), LS2 ([NH₄NO₃] = 0, [KNO₃] = 950 mg/L), LS3 ([NH₄NO₃] = 16.5, [KNO₃] = 1900 mg/L), LS4 ([NH₄NO₃] = 0, [KNO₃] = 2850 mg/L)] containing 2,4-D (10⁻⁶ M), kinetin (10⁻⁵ M) in the dark, (d) LS, mLS and vitamins-free media (LS* and mLS*). Except for the experiments of 3b, all the media applied in the other experiments contained 30 g/L of sucrose.

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