Detailed Investigations on the Solid Cell Culture and Antimicrobial Activities of the IranianArnebia euchroma

Figure 4

(a) The growth (filled bars) and the pigment production (spotted bars) of the A. euchroma callus on mLS medium containing 2,4-D (10⁻⁶ M), kinetin (10⁻⁵M), and stared media [LS, mLS, SH and White media containing IAA (10⁻⁶ M), kinetin (10⁻⁵ M)] in the dark. The pigment production of the A. euchroma callus subcultured (b) on White medium containing IAA (10⁻⁶ M), kinetin (10⁻⁵ M), and different amounts of copper sulfate in the dark, (c) in dark on White and M9 media containing [W1&M9a (IAA 10⁻⁷ M, kinetin 10⁻⁵ M), W2&M9b (IAA 10⁻⁶ M, kinetin 10⁻⁵ M), W3&M9c (IAA 10⁻⁵ M, kinetin 10⁻⁵ M), W4&M9d (IAA 10⁻⁷ M, kinetin10⁻⁶ M), W5&M9e (IAA 10⁻⁶ M, kinetin 10⁻⁶ M), W6&M9f (IAA 10⁻⁵ M, kinetin 10⁻⁶ M)], (d) The amounts of the K⁺ and Na⁺ ions in White, M9, and mM9 media, (e) The growth of the selected bacteria in the liquid Mueller-Hinton (filled bars) containing THF (spotted bars), 200 μg/mL THF extract (dashed bars). All the media applied in the experiments of 4a-d contained 30 g/L of sucrose.

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