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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 165894, 6 pages
http://dx.doi.org/10.1155/2011/165894
Research Article

Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths: A Useful Resource for Comparative Genomics of Lepidoptera

1Insect Genome Research Unit, National Institute of Agrobiological Sciences, Owashi 1-2, Tsukuba, Ibaraki 305-8634, Japan
2Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
3Entomology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8605, Japan
4Laboratory of Applied Molecular Entomology, Research Institute of Agriculture, Hokkaido University, N9, W9, Kita-ku, Sapporo 060-8589, Japan

Received 30 June 2010; Accepted 25 October 2010

Academic Editor: Xin-yuan Guan

Copyright © 2011 Yuji Yasukochi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, 𝑛 = 3 1 , which are not closely related with each other or with the silkworm, Bombyx mori, ( 𝑛 = 2 8 ), the sequenced model lepidopteran. A total of 108–184 clones representing 101–182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species. The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.