Figure 3: Flow cytometric analyses of iDC and mDC generated from PBMC of the same donor using the FastDC and 7-day culture methods. PBMC freshly prepared from one donor were analysed before culture and then were cultured for DC generation using both the FastDC and 7-day methods. In FastDC culture cells were cultured in IL-4 and GM-CSF for first 24 hrs followed by another 24 hrs to mature DC in TNF-α, IL-1β, IL-6, and PGE2. In 7-day culture, PBMC were stimulated in IL-4 and GM-CSF at iDC stage for 5 days then with TNF-α for 2 days to obtain mDC. Data are representative of at least three samples. Unshaded histograms drawn with broken lines are isotype controls and shaded histograms represent marker expression.