Table 1: Matrix interference.

LL of estimated potencyEstimated potencyUL estimated PotencyLL of relative potency  (%)Relative potencyUL of relative potency


The table shows data from one concentration of IL-2, IL-6, IL-1β, or TNF-α. Wider concentrations of IL-2, IL-6, IL-1β and TNF-α found no interference with our assay (not shown in the table). The tested ranges are the following: IL-2: 200–2000 IU/mL; IL-6: 200–2000 IU/mL; IL-1β: 40–400 U/mL; TNF-α: 100–1000 ng/mL. The values in bold face represent relative potency compared with the reference standards as determined by statistical analyses. LL stands for lower limits of the 95% interval while UL denotes upper limits of the 95% interval.