Figure 4: (a) Partial amino acid sequences of CLN6 from a number of species showing uniqueness of the p.W277R mutation in the affected Australian Shepherd. (b) Partial amino acid sequence of human CLN6 showing missense mutation sites (shaded: p.M241T1; p.R252H2; pG259C3; p.P299L5; p.W300R6) that result in human vLINCL and the amino acid corresponding to the Australian Shepherd mutation (underlined: p.W277R4).