The Utilization of Triton X-100 for Enhanced Two-Dimensional Liquid-Phase Proteomics

Table 1

The summary of experimental and theoretical pI intervals of five proteins included in protein test mixture purchased from Beckman Coulter.

Lane no.^a	Protein band^b	Protein	MW (Da)	Theoretical pI interval	Experimental pI interval^c
2	A	CCK (cholecystokinin)	1055	<4.00	4.02–4.04
4	B	Trypsin inhibitor	20100	4.00–4.60	4.12–4.42
5	C	α-Lactalbumin	14200	4.50–5.20	4.42–4.72
10	D	Insulin	5808	5.90–6.60	5.74–6.04
18	E	Ribonuclease A	5500	>8.50	8.00–8.10

^a,b,cRefer to Figure 3(b).