Mouse distal chromosome 7 contains two complex imprinted loci. (a) A diagrammatic representation of mouse distal chromosome 7 with maternal inheritance indicated in red and paternal inheritance indicated in blue. Genes are represented by the black boxes with the parent-of-origin expression indicated by either a red (maternal) or blue (paternal) arrow. Biallelic expression is indicated by a black arrow. Noncoding RNAs are represented by dashed arrows. DNA methylation is represented by the black lollypops. Green boxes indicate position of ICs whilst green doubleheaded arrows indicate the region influenced by the IC. (b) BAC clones that are used to examine expression, imprinting, and function. Blue boxes indicate the position of lacZ insertion. (c) Images of transgenic embryos inheriting the modified BACs through either the maternal or paternal germline as indicated by gender symbols below image. Expression of Cdkn1c from the BAC is revealed by lacZ reporter expression.
