BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes

Figure 1

Histogram of relative fluorescence intensity (flow karyotype) obtained after the analysis of DAPI-stained suspension of mitotic metaphase chromosomes prepared from double ditelosomic line 7D. The inset shows examples of flow-sorted 7DL and 7DS telosomes after FISH with probes for the telomeric repeat (red color) and the GAA repeat (turqoise color), which were used to identify the telosomes in the sorted fractions and estimate their purities.