BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes

Table 2

Results of 7DS library screening with microsatellite markers Xcfd68, Xbarc214, and Xgwm473.

Marker	Xcfd68	Xbarc214	Xgwm473
Product size	207 bp	217 bp	220 and 226 bp	200 bp
Contig	ctg244	ctg148	ctg285	ctg135
No. of hit BAC clones^1	9	15	7 + 3^3	16
Total no. of positive clones^2	11	17	7 + 3^3	16

¹Positive clones identified by PCR screening only.
²Hit BAC clones plus clones identified with the use of contig assembly data.
³Three of ten BAC clones providing the 220 and 226 bp products were missing from the assembly, thus their location in ctg285 could not be confirmed.