Culture Environment-Induced Pluripotency of SACK-Expanded Tissue Stem Cells
Figure 3
Derivation of reprogrammed colonies by culturing mPanstra cells under pluripotent stem cell culture conditions. (a) mPanstra cells grown in their maintenance medium were transferred to monolayers of STO feeder cells in medium supplemented with dialyzed fetal bovine serum (DFBS), heat-inactivated fetal bovine serum (hiFBS), or KnockOut Serum Replacement (KOSR). Each type of serum supplement was evaluated with (Xn) or without (Ctrl) Xn supplementation. Only SACK-D strains mPanstra-12 and mPanstra-25 formed colonies. (b) SACK-D mPanstra strains (top panel, under routine culture conditions) generate two types of colonies two weeks after transfer into reprogramming conditions: striated (str, middle panels) or epithelial-like (epl, lower panels). (c) A subset of cells from epithelial colonies express alkaline phosphatase (red color, indicated by arrows), a general pluripotency marker. μm.
