Experimentally Approaching the ICU: Monitoring Outcome-Based Responses in the Two-Hit Mouse Model of Posttraumatic Sepsis

Figure 1

Schematic of the two-hit model and repetitive blood sampling. 3-month-old female mice were subjected to trauma and hemorrhage (TH) followed by polymicrobial CLP-induced sepsis. Time span of model was divided into a pre-and post-CLP phase. Starting at TH, a 20 μL blood sample was collected daily (until post-TH day 7 post-CLP/day 5) from each animal, including one extra sample at 6 h after-CLP.