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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 359042, 6 pages
Research Article

Improving Antigenicity of the Recombinant Hepatitis C Virus Core Protein via Random Mutagenesis

1Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30010, Taiwan
2Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung 81143, Taiwan

Received 14 March 2011; Accepted 10 May 2011

Academic Editor: Shahid Jameel

Copyright © 2011 Chen-Ji Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

For improving the antigenicity of HCV core antigen, random mutations of this recombinant antigen were generated and evaluated by reverse-ELISA. Several mutants with higher antigenicities were examined. They can be used to develop a highly sensitive HCV ELISA. Following supplemental material provides additional information for the strategy of antigen screening (Figure S1), the antigen’s sequence identity (Figure S2), the antigen's purity (Figure S3), and the plot of double-reciprocals for kinetic constants calculation (Figure S4). More detail and important data please refer to the original texts.

  1. Supplementary Material