Chronic Heat Stress Weakened the Innate Immunity and Increased the Virulence of Highly Pathogenic Avian Influenza Virus H5N1 in Mice
Figure 3
Effects of CHS on the virulence of HPAIV in the infected mice. (a) Six mice per group were observed for mortality at 14 days. And the viral load in lungs at day 3 postinfection was determined by real-time PCR (b) and plaque assay (c).(d) The mRNA levels of IL-6, IFN-β, IL-10, and HSP70 in the lung at day 3 postinfection were determined by qPCR. * indicates when compared with the TN group. (e) Representative lung sections from each group were stained with immunohistochemical staining with an antiinfluenza NP mAb (400×), and the arrow indicated the NP positive cells.
