Figure 1: Hematocrit increase is directly reflective of Epo dose treatment. C57BL/6 male mice (6 wks of age) were put on 60% high fat diet and received exogenous Epo treatment of the following doses (1000, 600, 300, 150, and 75 U/kg) injected subcutaneously, three times a week; high-fat diet and control diet fed mice injected with saline only were used as sham and normal diet controls, respectively (  mice/group). At the end of the study (i.e., week 5), hematocrit was measured for all mice, and the results are expressed as the mean of final % hematocrit ± SEM. ANOVA single factor test was used for statistical analysis; values of were considered statistically significant.