Clinical Cancer Therapy by NK Cells via Antibody-Dependent Cell-Mediated Cytotoxicity
Figure 1
Immunocytokine facilitates adhesion of NK cells to tumor cells. This figure, adapted from Buhtoiarov et al. [36], shows the adhesion of M21 human melanoma tumor cells to NKL human NK cells after coincubation for 30 minutes. Tumor cells were prelabeled with CFSE, and NK cells were prelabeled with BODIPY and coincubated in medium alone, with hu14.18 (mAb) alone, soluble IL2, hu14.18 + soluble IL2, or hu14.18-IL2 immunocytokine (IC). The percentage of cells that had formed conjugates was defined as double positive cells as determined by flow cytometry.
