Combined Reversed Phase HPLC, Mass Spectrometry, and NMR Spectroscopy for a Fast Separation and Efficient Identification of Phosphatidylcholines

Table 2

Identified PC compounds within extracts of bovine brain, egg yolk, and soy bean.

Fatty acid position		ECN	Bovine brain	Egg yolk	Soy bean
sn-1 position	sn-2 position
Myristic acid(14 : 0)	Palmitic acid (16 : 0)	30	+	−	+
Palmitic acid (16 : 0)	Palmitoleic acid (16 : 1)	30	+	+	+
Palmitic acid (16 : 0)	Linoleic acid (18 : 2)	30	+	+	+
Palmitic acid (16 : 0)	Linolenic acid (18 : 3)	28	−	+	−
Palmitic acid (16 : 0)	Arachidonic acid (20 : 4)	28	+	+	+
Palmitic acid (16 : 0)	Palmitic acid (16 : 0)	32	+	+	+
Palmitic acid (16 : 0)	Oleic acid (18 : 1)	32	+	+	+
Stearic acid (18 : 0)	Linoleic acid (18 : 2)	32	+	+	+
Stearic acid (18 : 0)	Arachidonic acid (20 : 4)	30	+	−	+
C18 : 0 Plasmalogen	Oleic acid (18 : 1)	34	+	−	−
Palmitic acid (16 : 0)	Stearic acid (18 : 0)	34	+	−	−
Stearic acid (18 : 0)	Oleic acid (18 : 1)	34	+	+	+
Stearic acid (18 : 0)	Eicosenoic acid (20 : 1)	36	+	−	−