Both Basic and Acidic Amino Acid Residues of Are Involved in Triggering Substate of RyR1
Figure 3
Effects of IpTxa on purified rabbit RyR1 incorporated in planar lipid bilayers. (a) Single channel activity of purified RyR1 incorporated in planar lipid bilayers at 10 μM Ca2+ with or without IpTxa (6–100 nM) was recorded. Single channel currents are shown as downward deflections from the closed level (indicated by c). The holding potential was −30 mV. (b) The plot of of purified RyR1 versus various concentrations of IpTxa is shown. (c) The plot of of purified RyR1 versus various concentrations of IpTxa is shown. The results are shown as the means ± SE for 7 experiments.
