Adherent Primary Cultures of Mouse Intercostal Muscle Fibers for Isolated Fiber Studies

Figure 7

NFATc1-GFP distribution in intercostal and FDB fibers. FDB (a–c) and intercostal (d–f) fibers expressing NFATc1-GFP imaged at rest after 3 days in culture. Scale bar, 5 μm. Nuclei in intercostal fibers exhibit significantly elevated levels of NFATc1 when compared to FDBs cultured under the same conditions (g), N/C ratio of NFATc1-GFP in ITC = 1.38 ± 0.05, 𝑛=102 nuclei versus 0.51 ± 0.01, 𝑛=42 nuclei, in FDB fibers, 𝑃<0.05). Fluorescence was detected as in Figure 6.

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