Exploring Airway Diseases by NMR-Based Metabonomics: A Review of Application to Exhaled Breath Condensate
Figure 2
Representative one-dimensional 1H-NMR spectra from different patients. Spectra of saliva (left traces) and EBC samples (right traces) from a healthy (HS, lower traces), a laryngectomized (middle spectra), and a COPD (top spectra) subject are reported. Spectra were recorded on a Bruker Avance spectrometer operating at a frequency of 600.13 MHz (1H) and equipped with a TCI CryoProbe. The water resonance was suppressed by using a specific pulse sequence designed to avoid intensity alteration of signals. The total acquisition time was ca. 10 minutes per sample. Spectra were referred to TSP assumed to resonate at = 0.00 ppm. In saliva spectra, the group of signals centered at 3.8 ppm originates from carbohydrates, and is not visible in the corresponding EBC spectra.
