Cloning and Expression of Highly Pathogenic Avian Influenza Virus Full-Length Nonstructural Gene in Pichia pastoris

Table 1

OD₆₅₀ values of preliminary ELISA for detection of positive NS1 recombinant protein.

Dilution	10	20	40	80	160	320	640	1280	2560	5120	10240	Control
*Positive	0.924	0.945	0.865	0.729	0.597	0.489	0.325	0.245	0.127	0.096	0.078	0.054
**Negative	0.083	0.080	0.072	0.059	0.051	0.047	0.034	0.030	0.031	0.026	0.334	0.032

*Positive purified cell lysate. **Negative purified cell lysate.