HDAC Activity Is Required for Efficient Core Promoter Function at the Mouse Mammary Tumor Virus Promoter
HDAC inhibition does not affect the stability of initiation complexes at the MMTV promoter as determined by template competition assay. Transcription of the MMTV template in extracts from control or TSA-treated cells was carried out in the presence of increasing amounts of AdML template. A representative experiment is shown in (a). (b) shows the relative level of MMTV transcription in extracts from either control or TSA-treated cells. Transcriptional levels in each extract are expressed relative to those measured in the absence of AdML template. The results shown are derived from at least 3 independent experiments. The data were subjected to linear regression analysis to generate lines and slopes using GraphPad Prism. Error bars represent SEM.