Figure 2: In vitro motility speeds at pCa 5 (solid bars) or pCa 9 (open bars) for regulated thin filaments (α-GS-Tm WT, α-GS-Tm V95A, α-GS-Tm D175N, α-GS-Tm E180G, or β-GS-Tm WT and HcTn), or at pCa 5 (solid bars) or in actin buffer (AB)-based motility buffer (pCa ~9; see Section 2.5) for unregulated F-actin. Values are the weighted mean + S.D. for 4–10 flow cells (weighted by the number of filament paths tracked in each flow cell).