Figure 5: Temperature dependence of sliding speed at pCa 5 for regulated thin filaments reconstituted with HcTn and either α-GS-Tm E180G (■) or α-GS-Tm WT (○) [note that the control WT data are a subset of regulated thin filament data replotted from [42]]. Data were obtained during the heating phase of two cycles of heating and cooling in flow cells configured with a microfabricated thermo-electric heater for continuous variation of temperature (see Section 2). Points represent mean of the sliding speed for 3–8 filaments during one second. Note the deviation of mutant Tm data from WT as temperature increases.