Ketamine Inhibits Lung Fluid Clearance through Reducing Alveolar Sodium Transport
Figure 2
Patch clamp recordings of amiloride-sensitive currents in A549 cells. (a) Representative recordings of amiloride-sensitive current traces in a cell before (control) and after delivery of different concentrations of ketamine (30 μM to 1 mM) to the bath. (b) Concentration-dependent inhibition of whole-cell currents by ketamine. For each cell, inward currents recorded at −100 mV were measured during perfusion with external solutions. Amiloride-sensitive (AS) currents were normalized to the current at −100 mV of control. IC50 was calculated by best fitting the raw data points to the Hill equation. Inorm = 1/(1 + (ketamine/IC50))coe. (c) Amiloride-sensitive current density- (ASIdensity-) voltage curves for average control currents (control), in the presence of different concentrations of ketamine (30 μM to 1 mM). Average values are presented as the mean ± SEM. One-way ANOVA. *.
