Neurotrophic Features of Human Adipose Tissue-Derived Stromal Cells: In Vitro and In Vivo Studies
Figure 2
In vitro neurotrophic effects of ATSC. LAN-5 human neuroblasts and PC12 rat cells were cultured either ATSC-conditioned medium or co-cultured with ATSC and morphological modifications were monitored over time: (a) LAN5 in standard culture medium; (b) LAN5 cultured in ATSC-CM for 72 hours; (c) and (d) LAN-5 co-cultured with ATSC using a cell density of 104 cell/cm2 for both cell populations; (e) PC12 in standard culture medium; (f) PC12 cultured in ATSC-CM for 4 days; (g) PC12 cultured in βNGF 100 ng/mL for 4 days; (h) PC12 cultured in HDF-conditioned medium for 4 days. Arrows show evidence of neurite outgrowth; asterisk (*) indicate ATSC in culture. Scale bar 100 μm in all panels except for panel μm.