Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos
Figure 3
Integrin clustering is impaired in zebrafish embryos depleted of obscurin A. Zebrafish embryos were injected with GFP-5-integrin mRNA and then immunolabeled with anti-GFP (green: (a), (c), (e)) and antifibronectin (red: (b), (d), (f)) antibodies at 18 hours after fertilization (hpf). (a), (b) In control embryos, fibronectin matrix is organized at sites where 5-integrin has clustered along a nascent somite boundaries (arrows). (c)–(e) In mildly affected (c), (d) and more markedly affected (e), (f) obscurin-A-depleted embryos, there is reduced apical clustering of integrins along nascent boundaries, although in the few instances where there is significant integrin clustering, fibronectin is organized ((c), (d): arrows). Scale bars are 50 μm.
